|Year : 2016 | Volume
| Issue : 1 | Page : 43-46
Quality control of an antipsoriatic ayurvedic herbal Formulation: Lajjalu Keram
MT Athar1, SM Musthaba2, AK Al-Asmari3, S Baboota2, J Ali2, Sayeed Ahmad4
1 Department of Pharmacognosy and Phytochemistry, Hamdard University, New Delhi, India; Department of Research, Prince Sultan Military Medical City, Riyadh, KSA
2 Department of Pharmaceutics, Hamdard University, New Delhi, India
3 Department of Research, Prince Sultan Military Medical City, Riyadh, KSA
4 Department of Pharmacognosy and Phytochemistry, Hamdard University, New Delhi, India
|Date of Web Publication||13-Apr-2016|
Department of Pharmacognosy and Phytochemistry, Hamdard University, New Delhi
Source of Support: None, Conflict of Interest: None
Background: Psoriasis is an autoimmune disorder, which affects a large group of human population of world (3%). Till date, there is no treatment for psoriasis except some herbal drugs and its constituents. Since Ayurveda is the main traditional system of medicine in India, here, we have selected one ayurvedic formulation - Lajjalu Keram, which has been used since long for their quality control. Methods: Total microbial load of formulations were carried out for total fungal count and total bacterial count. Lajjalu Keram was also tested by high-performance liquid chromatographic (HPLC) for aflatoxins (B1, B2, G1, and G2), which showed its presence below the permissible limit; similarly, pesticides residues were analyzed using gas chromatography/mass spectrometry for organophosphates and organochlorides, which showed that pesticides were below detection limit (0.1 ppb). The content of heavy metals was analyzed using AAS, which demonstrated the presence of cadmium, lead, and arsenic below permissible limit, whereas mercury was found absent. Results: The result of quality control analysis showed the presence of alkaloids, tannins, carbohydrate, saponins, proteins and amino acids, lipid/fats, phenolic compounds, and flavonoids in formulation. The dermal toxicity (LD50) of Lajjalu Keram in Wistar rats was found more than 2000 mg/kg (safe for the management of psoriasis). Formulation was also analyzed for their composition of fatty acids. It was found to have 13 fatty acids, out of which, seven were saturated fatty acids (95.2%) and the rest were unsaturated fatty acids (3.27%). A rapid HPLC method for quantification of mimosine (an unusual amino acid present in formulation) has been developed and validated. The mimosine content in Lajjalu Keram was found to be 0.0070% w/w with % relative standard deviation of 0.41. Conclusion: The formulation afforded significant and better protection of carrageenan-induced rat paw edema (72.11% inhibition) as compared to control. The shelf life studies on antipsoriatic herbal formulations were carried out for 6 months at different time intervals. No significant variation in analysis parameters was observed on the storage of formulations up to 6 months. The assay of constituents using mean curve of sigma plot showed 44.2 month of shelf life for Lajjalu Keram.
Keywords: Aflatoxin, gas chromatography-mass spectrometry, high-performance liquid chromatographic, Lajjalu Keram, quality control
|How to cite this article:|
Athar M T, Musthaba S M, Al-Asmari A K, Baboota S, Ali J, Ahmad S. Quality control of an antipsoriatic ayurvedic herbal Formulation: Lajjalu Keram. Drug Dev Ther 2016;7:43-6
|How to cite this URL:|
Athar M T, Musthaba S M, Al-Asmari A K, Baboota S, Ali J, Ahmad S. Quality control of an antipsoriatic ayurvedic herbal Formulation: Lajjalu Keram. Drug Dev Ther [serial online] 2016 [cited 2017 Mar 24];7:43-6. Available from: http://www.ddtjournal.org/text.asp?2016/7/1/43/180162
| Introduction|| |
Psoriasis is an autoimmune disorder, which affects a large group of human population of world (3%). Till date, there is no treatment for psoriasis except some herbal drugs and its constituents. Since Ayurveda is the main traditional system of medicine in India, here, we have selected one ayurvedic formulation - Lajjalu Keram, which has been used since long for the management of psoriasis.
| Materials and Methods|| |
Standardization and phytochemical investigation
Lajjalu Keram was standardized as per standard protocol (WHO and AYUSH). Phytochemical screening was performed as per I.P. 1996, by taking whole extract. Results are summarized in [Table 1].
|Table 1: Results of various standardization parameters for quality control of Lajjalu Keram|
Click here to view
Chemical toxicity evaluation
Extraction of Aflatoxin was carried out as per AOAC official method. The extracted aflatoxin was de-rivatized and analyzed by high-performance liquid chromatographic (HPLC) (Shimadzu, Japan; Column: C18 15 cm × 4.6 mm; flow rate: 1 mL min −1; Solvent: Water/Acetonitrile/Methanol: 70/17/17; Detector: Fluorescent detector [Table 1].
Extraction of pesticide was also carried out as per AOAC official method. The formulation was dried in rotary evaporator (Buchi, Lab-scale Rotavapor ® R-210/R-215) and concentrated up to 5 mL and analyzed by gas chromatography (GC)-mass spectrometry (Perkin Elmer Clarus 500) [Table 1].
The formulation was incinerated in a silica crucible (Temp: 600°C). The ash obtained was dissolved in Aquaragia and analyzed by Atomic Absorption Spectrometer (Light Source: Hallow cathode lamp; Flame: Air/Acetylene) [Table 1].
Preclinical toxicity evaluation
Preclinical toxicity was performed on Wistar rats according to OECD protocol (for 14 days). Histopathological studies were carried out [Figure 1]a, [Figure 1]b,[Figure 1]c,[Figure 1]d,[Figure 1]e,[Figure 1]f,[Figure 1]g,[Figure 1]h.
|Figure 1: Photomicrograph of sections of skin of different groups showing a normal histological appearance of epidermis and dermis at low resolution (×100) and high resolution (×400). (a) control female group (×100); (b) control female group (×400); (c) control male group (×100); (d) control male group (×400); (e) female rat group treated with Lajjalu Keram (×100); (f). female rat group treated with Lajjalu Keram (×400); (g) male rat group treated with Lajjalu Keram (×100); (h) male rat group treated with Lajjalu Keram (×400). (i) Anti-inflammatory activity of Lajjalu Keram against carrageenan-induced paw edema showing percentage (%) inhibition of paw edema|
Click here to view
Analysis of fatty acid constituents
Fatty acid analysis of formulation was performed by gas chromatography/flame ionization detector GC/flame ionization detector (FID) equipped with autosampler (Perkin Elmer Clarus 500; Column: Supelcowa × 10, 30 m × 0.25 mm; film thickness 0.25 µm; Carrier gas: H2; 10 psi; Detector: FID; detector temp: 290°C; oven temperature: 130°C for 5 min and raised @ 4°C/min to final temperature of 240°C for 12.5 min; Injector temperature: 260°C; Injection volume: 1.5 µL) [Figure 2]a.
|Figure 2: (a) Gas chromatography-flame ionization detector chromatogram showing different types of fatty acids present in Lajjalu Keram (b): Overlay high-performance liquid chromatographic chromatograms showing the presence of mimosine in Lajjalu Keram coinciding with peak of standard mimosine|
Click here to view
Analysis of mimosine in Lajjalu Keram
Mimosine was analyzed using a reversed-phase column chromatography (Shimadzu HPLC with quaternary LC-10A VP pumps; C18 reversed phase Phenomenex column; ultraviolet (UV)-visible detector, Class-VP 5.032 software). The mobile phase was water: Orthophosphoric acid (98.8:0.2, v/v; pH 3.0). Mimosine was eluted isocratically with a flow rate of 1.0 mL min −1. The eluate mimosine was monitored by UV detector at a wavelength of 284 nm). The content of mimosine in the formulation was analyzed [Table 1] and [Figure 2]b.
Anti-inflammatory activity was carried out against carrageenan-induced rat paw edema as per the method described by Balian using digital plethysmometer. Paw volume was recorded immediately before carrageenan injection and regarded as initial paw volume. Further, paw volume was recorded at the durations of 1, 2, 4, 6, 12, and 24 h after carrageenan injection [Figure 1]i. Percentage inhibition of edema was calculated by using the following formula:
Shelf life determination
Shelf life of Lajjalu Keram was determined by assaying the content of mimosine at different time intervals up to 6 months after storing the drug on shelf at room temperature. Other organoleptic properties (physical appearance, color, odor, and taste), acid value, viscosity and GC fingerprint were also analyzed [Table 2]. The assay data were further evaluated using Sigmaplot ™ 11 (Cranes software International, Bangalore, India) to calculate the shelf life of Lajjalu Keram.
|Table 2: Stability studies of Lajjalu Keram at 0, 3, and 6 months for determination of shelf life|
Click here to view
| Results and Discussion|| |
The Lajjalu Keram was studied for standardization as per WHO and AYUSH guidelines. The results of phytochemical analysis confirm the presence of alkaloids, saponin, lipids, phenolic, tannins, and carbohydrate and flavonoid compounds. In this study, absence of total bacterial count has been observed. However, there was presence of colonies forming units with the fungus media. The important finding including heavy metals, aflatoxins, and pesticides were determined to ensure the safety of the drug intended for human use and were found that all are within limit. The finding can be further used as QC tool for formulation evaluation.
Preclinical toxicity studies revealed the safety potential of the formulation because no mortality was observed in the treated (up to 5000 mg kg −1 of BW) and control group of animals. No visible signs of treatment (i.e., changes in respiration, circulatory, nervous system, and behavioral pattern) were observed. Necropsy examination did not expose abnormal lesion in any group.
Analysis of fatty acids confirms the presence of fatty acids (total: 98.47%). There were 13 fatty acids detected in the formulation, out of which, seven were saturated fatty acids (95.2%) and the rest were unsaturated fatty acids (3.27%). Among unsaturated fatty acids, maximum amount was of three types of dienoic fatty acid (2.53%).
An rapid HPLC method for quantification of mimosine in the Lajjalu Keram has been developed and validated for the first time for quality control of Lajjalu Keram. The developed and validated method was applied for the analysis of mimosine in Lajjalu Keram. The mimosine content in Lajjalu Keram was found to be 0.0070% w/w with % relative standard deviation of 0.41. The method can be used for routine quality control analysis of Lajjalu Keram because of wide range of linearity, simple mobile phase, UV detection, lack of extraction procedure, low RT, and without using any internal standard.
The formulation afforded significant protection of carrageenan-induced rat paw edema (72.11%) as compared to control. The shelf life studies on antipsoriatic herbal formulations were carried out for 6 months. The assay of constituents using mean curve of sigma plot showed 44.2 months of shelf life Lajjalu Keram.
| Conclusion|| |
In brief, the quality control assessment and chromatographical analysis of Lajjalu Keram formulation was performed. The studied formulation was found safe since the absence of pesticides and limit presence of heavy metals and aflatoxins. As no mortality was observed during the study, it can be revealed that the extract could be used as a potential antipsoriatic agent.
Financial support and sponsorship
We would like to thank CCRAS, AYUSH, Government of India for financial assistance.
Conflicts of interest
There are no conflicts of interest.
| References|| |
Horwitz W. Official Method of Analysis of the Association of Official Analytical Chemists. 11th
ed. Washington, DC: AOAC; 1970.
Musthaba SM, Athar MT, Kamal YT, Ahmad S, Ali J, Baboota S. Quantitative estimation of mimosine in anti-psoriatic ayurvedic formulation containing whole plant extract of Mimosa pudica
by validated isocratic RP-HPLC method. Acta Chromatogr 2011;23:531-8.
Balian S, Ahmad S, Zafar R. Anti-inflammatory activity of leaf and leaf callus of Silybum marianum
(L.) in albino rats. Indian J Pharmacol 2006;38:213-4.
[Figure 1], [Figure 2]
[Table 1], [Table 2]