Drug Development and Therapeutics

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 7  |  Issue : 2  |  Page : 87--91

Characterization of Arachis hypogaea L. oil obtained from different extraction techniques and in vitro antioxidant potential of supercritical fluid extraction extract


Rishika Chauhan, Iftekhar Ahmad, Yasmeen Khan, Ennus Tajuddin Tamboli, Sayeed Ahmad 
 Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi, India

Correspondence Address:
Sayeed Ahmad
Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi - 110 062
India

Aim: The present investigation was aimed to characterize the fixed oil of Arachis hypogaea L. using five different extraction methods: Supercritical fluid extraction (SFE), ultrasound assistance extraction, soxhlet extraction, solvent extraction, and three phase partitioning method. Materials and Methods: The SFE conditions (temperature, pressure, and volume of CO 2) were optimized prior for better yield. The extracted oils were analyzed and compared for their physiochemical parameters, high-performance thin layer chromatography (HPTLC), gas chromatography-mass spectrometry (GC-MS), and Fourier transform infrared spectrometry (FT-IR) fingerprinting. Anti-oxidant activity was also determined using DPPH and superoxide scavenging method. Results: The main fatty acids were oleic, linoleic, palmitic, and stearic acids as obtained by GC-MS. HPTLC analysis revealed the presence of similar major components in chromatograms. Similarly, the pattern of peaks as obtained in FT-IR and GC-MS spectra of same oils by different extraction methods was superimposable. Conclusion: Analysis reported that the fixed oil of A. hypogaea L. is a good source of unsaturated fatty acid, mainly n-6 and n-9 fatty acid with a significant antioxidant activity of oil obtained from SFE extraction method.


How to cite this article:
Chauhan R, Ahmad I, Khan Y, Tamboli ET, Ahmad S. Characterization of Arachis hypogaea L. oil obtained from different extraction techniques and in vitro antioxidant potential of supercritical fluid extraction extract.Drug Dev Ther 2016;7:87-91


How to cite this URL:
Chauhan R, Ahmad I, Khan Y, Tamboli ET, Ahmad S. Characterization of Arachis hypogaea L. oil obtained from different extraction techniques and in vitro antioxidant potential of supercritical fluid extraction extract. Drug Dev Ther [serial online] 2016 [cited 2017 Mar 25 ];7:87-91
Available from: http://www.ddtjournal.org/article.asp?issn=2394-6555;year=2016;volume=7;issue=2;spage=87;epage=91;aulast=Chauhan;type=0